Immunoprecipitation (IP) of total HSV-2 antigen with 0ΔNLS antiserum pulled down 19 viral proteins. Many antibodies were directed against infected-cell proteins of >100 kDa in size, and only 10 ± 5% of antibodies were directed against gD. Western blot analyses indicated the live HSV-2 0ΔNLS vaccine elicited an IgG antibody response against 9 or more viral proteins. In light of these results, we sought to determine which viral proteins were the dominant antibody-generators (antigens) of the live HSV-2 0ΔNLS vaccine. We have observed that mice immunized with a live HSV-2 ICP0 - mutant virus, HSV-2 0ΔNLS, are 10 to 100 times better protected against genital herpes than mice immunized with a HSV-2 gD subunit vaccine ( PLoS ONE 6:e17748). Search HSWB or HSWBP code in the online Laboratory Test Catalog.Virion glycoproteins such as glycoprotein D (gD) are believed to be the dominant antigens of herpes simplex virus 2 (HSV-2). For infants with suspected HSV disease, viral culture and PCR tests should be performed. HSV IgM antibody is present during both primary and recurrent infections making result interpretation difficult. This Western blot assay detects IgG antibody. Call Reference Laboratory Services if you have further questions, (800) 713-5198. If the antibody subtype is unclear (about 20% of specimens), the serum will be adsorbed against HSV-1 and HSV-2 proteins and re-tested. Most results are available within five days. HSV Western blot serologies are run three times a week. Since fewer than 5% of patients demonstrate a detectable rise in antibody titer during recurrent HSV episodes, an interpretive report is given instead of numerical values. In addition, Western blot readily documents seroconversion. The specificity of the Western blot assay provides an accurate distinction in 99% of patients between antibody to HSV-1 and antibodies to HSV-2. Our laboratory detects HSV Antibodies by Western blot assay, which is not only highly sensitive for detecting HSV antibodies, but also is highly accurate in differentiating past HSV-1 from HSV-2 infections and determining whether someone has antibodies to both viruses. However, serologies for HSV are useful in determining whether a person has had a past infection with HSV-1 or HSV-2, and is the best way to detect “silent carriers” of HSV-2. Viral isolation and subsequent subtyping is generally the best way to document an acute HSV infection. Search HBCA code in the online Laboratory Test Catalog for information on Hepatitis B Core Antibody (HBcAb). Search HBSA code in the online Laboratory Test Catalog for information on Hepatitis B Surface Antibody (HBsAb). Anti-HBs assays and anti-HBc assays are performed daily Monday through Friday. Vaccination/re-vaccination is warranted with values below this level. of Hepatitis B surface antibody are considered protective levels. Values above a test standard containing 10 I.U. The Hepatitis B surface antibody result is reported international units (I.U.) of Hepatitis B surface antibody. needle stick exposures), for documenting persons with past infection with Hepatitis B virus, for vaccine screening, and for determining response to vaccination. Hepatitis B surface antibody (anti-HBs) and Hepatitis B core antibody (anti-HBc) assays are useful for identifying persons susceptible to Hepatitis B infection (i.e. See Hepatitis B Chart 1, Chart 2, and Chart 3 Search HBB code in the online Laboratory Test Catalog for more information on Hepatitis B Battery (HBSAb, HBSAg, and HBCAb). Search HBSS code in the online Laboratory Test Catalog for more information on Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb). Search HBSAG or HBSAGX code in the online Laboratory Test Catalog. Note: Positive HBsAg will reflex to a Hepatitis B DNA by PCR at an additional charge. The physician must also report acute Hepatitis B infections to the local County Department of Public Health (in King County, telephone the Communicable Disease Department at (206) 296-4774). The routine HBsAg run can accommodate most specimens generated by accidental parenteral exposure. Patients with needle sticks and other parenteral exposures need to receive Hepatitis B Immune Globulin within 72 hours of exposure. Reports of reactive HBsAg are called to the ordering physician or laboratory and are automatically run for Hepatitis B viral DNA by PCR. Infectivity of a patient is determined by enzyme immunoassay for Hepatitis B surface antigen (HBsAg), which is run Monday through Friday. Hepatitis B is transmitted through blood or secretions of infected patients.
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